An Unbiased View of PP88
An Unbiased View of PP88
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In a specific embodiment, stated presented impact on stated focused receiver bacterial cell generates, immediately or indirectly, a reaction in claimed organism internet hosting stated specific receiver bacterial cell.
Donor Bacterial Cell inside the context of the applying, the term “donor bacterial cell” refers to a bacterial mobile internet hosting a vector or simply a plasmid, to the production cell line or to your bacterium that's able to transferring a conjugative plasmid to a different bacterium.
considering that stated conditional origin of replication is inactive while in the qualified receiver bacterial mobile due to the absence of explained provided protein, peptid, RNA, nucleic acid, molecule or any mixture thereof in stated receiver bacterial mobile, claimed conditional origin of replication could possibly be picked based on the unique receiver bacterial cell to get focused.
By “in trans” is meant herein that said protein, peptid, RNA, nucleic acid, molecule or any mix thereof just isn't encoded on precisely the same nucleic acid molecule as the a single comprising the origin of replication. In a particular embodiment, said protein, peptid, RNA, nucleic acid, molecule or any combination thereof is encoded over a chromosome or with a plasmid.
By “modulating the microbiome” is meant herein exerting a modifying or controlling influence over the microbiome. inside the context of the invention, modulating the microbiome encompases modulating the microbiome functionality and/or modulating the microbiome composition.
Most preferably, the genetic modification isn't 立即獲得獎勵 going to entail possibly NHEJ or HR endogenous restore mechanism of your host microorganisms.
In a particular embodiment, antibiotic resistant strains are targetly killed by programming the nuclease to complete a DNA cleavage, e.
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In an alternative embodiment, said vector could be a plasmid, additional notably a conjugative plasmid, and explained donor bacterial mobile could then be a bacterium that may be capable of transferring mentioned conjugative plasmid to a different bacterium, specifically by conjugation.
Several clones ended up sequenced to verify which the p1319 plasmid contained no p15a-centered origin and that they also contained an intact primase gene by having an RBS coming through the library.
to be a Command for unspecific killing, packaged phagemids ended up also transduced in the pressure s11983, that is a by-product of the O157 H10dstx pressure missing the lacZ gene.
They are usually employed to select for recombination occasions amongst a plasmid carrying this sort of origins and the genome of the bacteria of fascination.
260、细菌噬菌体可选自肌尾噬菌体科(非限制性地比如以下属：cp220病毒、cp8病毒、ea214病毒、felixo1病毒、moogle病毒、susp病毒、hp1病毒、p2病毒、kay病毒、p100病毒、silvia病毒、spo1病毒、tsarbomba病毒、twort病毒、cc31病毒、jd18病毒、js98病毒、kp15病毒、moon病毒、rb49病毒、rb69病毒、s16病毒、schizot4病毒、sp18病毒、t4病毒、cr3病毒、se1病毒、v5病毒、abouo病毒、agate病毒、agrican357病毒、ap22病毒、arv1病毒、b4病毒、bastille病毒、bc431病毒、bcep78病毒、bcepmu病毒、biquarta病毒、bxz1病毒、cd119病毒、cp51病毒、cvm10病毒、eah2病毒、el病毒、hapuna病毒、jimmer病毒、kpp10病毒、m12病毒、machina病毒、martha病毒、msw3病毒、mu病毒、myohalo病毒、nit1病毒、p1病毒、pakpuna病毒、pbuna病毒、phikz病毒、rheph4病毒、rsl2病毒、rsluna病毒、secunda5病毒、sep1病毒、spn3病毒、svuna病毒、tg1病毒、vhml病毒和wph病毒)。
本发明涉及用于调节宿主微生物组的感兴趣的核酸，涉及编码所述核酸的载体以及涉及用于通过递送所述感兴趣的核酸来调节宿主微生物组的方法。
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